HotStart-IT®: A Novel Hot Start PCR Method Based on Primer Sequestration
نویسندگان
چکیده
منابع مشابه
Hot Start PCR with heat-activatable primers: a novel approach for improved PCR performance
The polymerase chain reaction (PCR) is widely used for applications which require a high level of specificity and reliability, such as genetic testing, clinical diagnostics, blood screening, forensics and biodefense. Great improvements to PCR performance have been achieved by the use of Hot Start activation strategies that aim to prevent DNA polymerase extension until more stringent, higher tem...
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The polymerase chain reaction (PCR) is an indispensable DNA amplification technique that has been exploited in numerous areas, including molecular diagnostics. One major drawback of PCR is the competing amplification of undesired off-target products, which primarily occurs at ambient temperatures prior to PCR cycling (1). Hot Start PCR techniques aim to block the extension of primers in nonspec...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2008
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000112827